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Expand Long Template Pcr System Manual. Expand Long Template PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages. Expand High Fidelity PCR. System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading
Expand Long. Template. Enzyme mix. • 30 l (150 U pack size). • 2 72 l (720 U pack size). • 10 72 l (3600 U pack size). Enzyme storage buffer: 20 mM Tris-HCl, pH 7.5. (25°C), 100 mM KCl, 1 mM dithiothreitol (DTT),. 0.1 mM .. 6 PCR Applications Manual, 2nd Edition. 7 PCR Product Family Flyer. 8 Lab FAQS “Find a Quick
Applications. PCR and DNA labeling reactions. Expand High Fidelity PCR System is especially optimized to efficiently amplify DNA fragments up to 5 kb. PCR is possible up to 9 kb with yield diminishing as DNA fragment length increases. For the generation of longer PCR products, the Expand Long Template. PCR System
For the generation of longer PCR products, the Expand Long Template. PCR System, which is optimized for the amplification of DNA frag- ments ranging from 3 kb to 27 kb in length, is recommended. Expand High Fidelity PCR System is composed of a special enzyme mix containing . 3 PCR Applications Manual.

Sigma-Aldrich offers Roche-ELONG-RO, Expand™ Long Template PCR System for your research needs. Purchase; Safety & Documentation Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity.
y Version 23. Content version: June 2011. Cat. No. 11 759 060 001. 3600 U (10 360 U) Application. Expand Long Template PCR System is a special enzyme
Protocol. AMPLIFICATION OF MITOCHONDRIAL DNA For amplification of mitochondrial DNA using the Expand Long Template PCR System refer to the following articles: Paul et al., Rapid mapping of mitochondrial DNA deletions by large-fragment PCR, Trends in Genetics Vol 12, p131, 1996; Ray et al., The spectrum of
or the MagNA Pure Compact Nucleic Acid Isolation Kit I (for auto- mated isolation) or. - the High Pure PCR Template Preparation Kit (for manual isolation). L Human genomic DNA and a human tPA Control Primer Set – especially tested with the Expand Long Range dNTPack - are available from Roche Applied Science as a
     

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